| 杨露,成涓,陈瑜莉,刘逢秋.光声/超声实时双模态显影纳米粒制备及示踪实验[J].中国介入影像与治疗学,2018,15(6):363-368 |
| 光声/超声实时双模态显影纳米粒制备及示踪实验 |
| Preparation of photoacoustic/ultrasonic real time dual-modality nanoparticles and imaging experiment |
| 投稿时间：2017-12-18 修订日期：2018-02-07 |
| DOI：10.13929/j.1672-8475.201710049 |
| 中文关键词: 光声成像 超声检查 造影剂 前哨淋巴结 |
| 英文关键词:Photoacoustic imaging Ultrasonography Contrast media Sentinel lymph node |
| 基金项目:国家自然科学基金青年科学基金（81701709）、重庆市科委基础科学与前沿技术研究一般项目（cstc2016jcyjA0295）、重庆市卫生和计划生育委员会面上项目（2016MSXM029）。 |
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| 中文摘要: |
| 目的 研制一种包裹纳米炭和液态氟碳的纳米粒（CNPs），并验证其光声/超声实时双模态示踪能力。方法 采用双乳化法制备CNPs。于光镜及透射电镜下观察并通过激光粒径仪检测CNPs的基本性质；以激光仪辐照CNPs，观察其液气相变情况；于激光共聚焦显微镜下观察荧光标记的CNPs被巨噬细胞吞噬的情况；激光辐照后观察CNPs体外增强光声/超声实时双模态显影情况；进行在体实验观察CNPs增强光声/超声实时双模态显影示踪VX2荷瘤兔腘窝转移淋巴结的效果。结果 成功制备CNPs，其平均粒径为（483.32±45.09）nm，Zeta电位为（-26.30±5.02）mV；且经激光辐照后，可发生液气相变；与巨噬细胞共孵育后，CNPs大量被巨噬细胞吞噬；CNPs在体外、体内均具有良好的光声显影与超声造影效果，且CNPs浓度越高，平均光声信号值及平均灰度值越大。结论 CNPs可在激光辐照后发生液气相变，具备光声成像与超声造影的实时双模态显影能力，并可在活体实验中示踪兔恶性肿瘤转移淋巴结。 |
| 英文摘要: |
| Objective To synthesize a kind of carbon nanoparticles incorporated liquid-gas phase-transition nanodroplets (CNPs), and to verify their traceability for photoacoustic/ultrasound dual-modality imaging at real time. Methods The nanodroplets were synthesized using dual-emulsion technique. The basic properties were observed and measured with optical microscope, transmission electron microscope and laser particle size analyzer. The phase transition of CNPs was observed after laser irradiation. DiI-labeled CNPs phagocytosed by macrophages were also observed with the laser scanning confocal microscope. The enhanced photoacoustic/ultrasound imaging with CNPs in vitro was observed after laser irradiation. And the in vivo experiment was used to analyze the effect of CNPs for tracking metastatic lymph nodes of the axilla in VX2 tumor-bearing rabbits with enhanced photoacoustic/ultrasound imaging. Results CNPs with the average diameter of (483.32±45.09)nm and Zeta potential of (-26.3±5.02)mV were successfully prepared. After laser irradiation, the phase-transition of CNPs obviously took place. After co-incubation with macrophages, CNPs were massively phagocytosed by macrophages. Photoacoustic and ultrasound imaging were significantly enhanced with CNPs in vitro and in vivo. And the mean photoacoustic signal and the grey level increased with the concentration of CNPs. Conclusion CNPs are successfully synthesized with excellent phase-transition feature after laser irradiation, which could be used for photoacoustic/ultrasound dual-modality imaging at real time. And this excellent property got further confirmed by tracking malignant metastatic lymph nodes in rabbits. |
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