| 方伟伟,成德雷,吕维富,朱楠,陈晓明,刘开才.大鼠下腔静脉型巴德-吉亚利综合征动物模型的制备[J].中国介入影像与治疗学,2018,15(12):743-747 |
| 大鼠下腔静脉型巴德-吉亚利综合征动物模型的制备 |
| Preparation of rat models of inferior vena cave-type Budd-Chiari syndrome |
| 投稿时间：2018-03-26 修订日期：2018-09-10 |
| DOI：10.13929/j.1672-8475.201803046 |
| 中文关键词: 巴德-吉亚利综合征 模型,动物 腔静脉,下 病理学 |
| 英文关键词:Budd-Chiari syndrome Models,animal Vena cava,inferior Pathology |
| 基金项目:安徽省自然科学基金项目（1708085QH218）。 |
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| 中文摘要: |
| 目的 通过部分结扎大鼠肝后段下腔静脉（IVC）建立IVC阻塞型巴德-吉亚利综合征（BCS）模型。方法 采用随机数字法将36只健康成年SD大鼠分为模型组和对照组。对模型组大鼠以3F微导管部分结扎肝后段IVC，对照组仅暴露肝后段IVC，其余处理相同。术后第28天取外周静脉血行肝功能检查，术后第84天和第85天分别行腹部超声和DSA，评价IVC阻塞程度及侧支静脉形成后处死大鼠，对肝脏、脾脏行病理学检查。结果 建模成功率83.33%（15/18）。术后第28天，模型组大鼠静脉血谷丙转氨酶、谷草转氨酶、碱性磷酸酶、谷氨酰转移酶和总胆红素均较对照组升高（P均< 0.05）；术后第84天和85天腹部超声及DSA检查均证实模型组大鼠存在IVC阻塞，肝内及肝外侧支静脉形成。光镜下模型组大鼠肝组织严重脂肪样变性，肝小叶中央静脉及其周围肝血窦扩张、淤血，汇管区纤维增生和轻度纤维化；电镜下可见肝细胞内细胞器减少，存在局限性水肿，胞质内大量脂滴堆积，粗面及细面内质网扩张，周围线粒体减少，肝细胞核膜裂隙增宽等改变。结论 部分结扎大鼠肝后段IVC可成功制备IVC阻塞型BCS，为BCS研究提供平台。 |
| 英文摘要: |
| Objective To establish animal models of inferior vena cava (IVC) type Budd-Chiari syndrome (BCS) through partial ligation of the posterior IVC of rats. Methods Thirty-six healthy adult SD rats were equally divided into model group and sham operation group using random number method. Retrohepatic IVC in model group were ligated with 3F microcatheter, while only isolated in sham operation group. Liver function was tested with peripheral venous blood on the 28th day after operation. Abdominal ultrasound and DSA were performed to evaluate the degree of IVC obstruction and the collateral vein formation on the 84th day and 85th day. The rats were then sacrificed, and the liver and spleen were pathologically examinated. Results The successful rate of model establishment was 83.33% (15/18). On the 28th day, alanine transaminase, aspartate aminotransferase, alkaline phosphatase, glutamyltransferase and total bilirubin in model group were higher than those in sham operation group (all P < 0.05). On the 84th day and 85th day, abdominal ultrasound and DSA showed IVC obstruction and the formation of hepatic and hepatic lateral branches in model group. Severe fatty degeneration of liver tissue, hepatic lobular central vein and surrounding hepatic sinusoid expansion and congestion, as well as mild fibrosis in the portal area were observed, while electron microscope showed decreased organelles, localized edema, accumulative lipid droplets in cytoplasm, expansive endoplasmic reticulum, reductive mitochondria and widening of nuclear membrane fissure in model group. Conclusion Rat models for IVC type BCS could be prepared through partial ligation of posterior IVC, therefore providing a platform for BCS researches. |
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