| 吴春华,杜伟,余义俊,王贝然.兔VX2肝癌经导管动脉栓塞术后癌旁肝组织氧化应激及细胞凋亡[J].中国介入影像与治疗学,2019,16(2):112-116 |
| 兔VX2肝癌经导管动脉栓塞术后癌旁肝组织氧化应激及细胞凋亡 |
| Oxidative stress and apoptosis of VX2 hepatocarcinoma aftertranscatheter arterial embolization in rabbit |
| 投稿时间：2018-04-15 修订日期：2018-10-25 |
| DOI：10.13929/j.1672-8475.201804022 |
| 中文关键词: 肝肿瘤 经导管肝动脉栓塞 氧化性应激 细胞凋亡 模型,动物 兔 |
| 英文关键词:liver neoplasms transcatheter arterial embolization oxidative stress apoptosis models,animal rabbits |
| 基金项目:国家自然科学基金（81660300）。 |
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| 中文摘要: |
| 目的 观察兔经导管动脉栓塞（TAE）治疗后VX2肝癌模型癌旁肝组织中氧化应激及细胞凋亡情况，探讨其在肝组织损伤中的作用。方法 建立25只兔VX2肝癌模型，随机分为TAE组（13只）及对照组（12只），对TAE组行TAE治疗，对照组不做任何处理。于TAE组术后3天及对照组同期检测外周血丙氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶（AST）、总胆红素（TBI）、血清白蛋白（ALB）、凝血酶原时间（PT）。而后处死实验动物，获取肿瘤组织及癌旁肝组织离体标本，用于组织病理检查、生物酶学检测及细胞凋亡检测。以生化酶学方法检测超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-PX）及过氧化氢酶（CAT）；采用TUNEL法检测肝细胞凋亡情况，计算细胞凋亡指数（AI）。结果 与对照组比较，TAE组ALT、AST、TBI升高，ALB降低，PT延长；SOD、GSH-PX、CAT均减低（P均<0.001）。TAE组癌旁肝组织可见轻度脂肪变性及大量炎性细胞浸润，对照组癌旁肝组织仅见轻度脂肪变性。TAE组AI为（64.20±2.77）%，明显高于对照组[（2.20±1.90）%；t=-112.30，P<0.001]。结论 兔VX2肝癌模型TAE术后肝功能下降可能与癌旁肝组织抗氧化指标活性明显降低，发生氧化应激，导致肝细胞凋亡增多有关。 |
| 英文摘要: |
| Objective To observe the oxidative stress and apoptosis of paracancerous liver tissue after transcatheter arterial embolization (TAE) in rabbit models with VX2 hepatocarcinoma, in order to investigate the role of oxidative stress and apoptosis in liver tissue injury. Methods Twenty-five rabbit VX2 hepatocarcinoma models were established and randomly divided into TAE group (n=13) and control group (n=12). TAE group was treated with TAE, while no treatment was performed on control group. Peripheral blood indexes, including alanine aminotransferase (ALT), aspartate aminotransferase (AST), total bilirubin (TBI), serum albumin (ALB) and prothrombin time (PT) were detected three days after operation in TAE group and the same time in control group. Then the experimental animals were killed, and the samples of tumor tissues and paracancerous liver tissues were obtained for histopathological examination, bioenzyme detection and apoptosis detection. The levels of superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), Catalase (CAT) were detected by biochemical enzymatic assay. The apoptosis of hepatocytes was detected by using TUNEL method, and the apoptotic index (AI) was calculated. Results Compared with control group, ALT, AST and TBI increased, ALB decreased and PT prolonged, while SOD, GSH-PX, CAT decreased in TAE group (all P<0.001). Mild steatosis and inflammatory cell infiltration were observed in paracancerous liver tissue of TAE group, while only mild steatosis was observed in control group. AI of TAE group was significantly higher than that of control group ([64.20±2.77]% vs[2.20±1.90]%; t=-112.30, P<0.001). Conclusion The decrease of liver function after TAE in rabbit VX2 hepatocarcinoma models maybe associate with the decrease of antioxidant activity and the oxidative stress which eventually lead to the increase of hepatocyte apoptosis. |
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