周頔,王志刚,文明,李咏梅,吕发金.制备靶向相变型载硫化铋纳米粒并用于体外CT/超声显像[J].中国介入影像与治疗学,2019,16(3):167-172
制备靶向相变型载硫化铋纳米粒并用于体外CT/超声显像
Preparation of folate-targeted phase-transition nanoparticles carrying bismuth sulfide and application in CT/ultrasound imaging in vitro
投稿时间:2018-09-20  修订日期:2019-01-01
DOI:10.13929/j.1672-8475.201809039
中文关键词:  造影剂  硫化铋  超声检查  体层摄影术,X线计算机
英文关键词:contrast media  bismuth sulfide  ultrasonography  tomography,X-ray computed
基金项目:国家自然科学基金面上项目(81171366)、国家自然科学基金青年科学基金项目(81801717)、重庆医科大学附属第一医院医学科学培育基金(PYJJ2018-15)。
作者单位E-mail
周頔 重庆医科大学附属第一医院放射科, 重庆 400016  
王志刚 重庆医科大学超声影像学研究所, 重庆 400010  
文明 重庆医科大学附属第一医院放射科, 重庆 400016  
李咏梅 重庆医科大学附属第一医院放射科, 重庆 400016  
吕发金 重庆医科大学附属第一医院放射科, 重庆 400016 fajinlv@163.com 
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中文摘要:
      目的 制备叶酸靶向相变型载硫化铋(Bi2S3)纳米粒(FBS-PFH-NPs)并用于体外细胞靶向及CT/超声显像。方法 采用旋转蒸发法和声振法制备FBS-PFH-NPs,检测其基本性质;以宫颈癌Hela细胞验证FBS-PFH-NPs体外寻靶能力;观察60、90、120、150、180 W功率HIFU辐照后FBS-PFH-NPs回声强度和温度变化,以及纳米粒中Bi2S3浓度为1.0、2.0、3.0、4.0、5.0 mg/ml时FBS-PFH-NPs体外CT及超声显像效果。结果 光镜下FBS-PFH-NPs呈球形,平均粒径(458.50±69.22)nm;Bi2S3均匀分布于其外壳,浓度为1.0 mg/ml。FBS-PFH-NPs大量结合于Hela细胞周围。HIFU辐照后,FBS-PFH-NPs发生液气相变,且随功率增高,FBS-PFH-NPs回声强度及温度均逐渐增高(F=110.09、440.69,P均<0.01)。随纳米粒中Bi2S3浓度增高,FBS-PFH-NPs的CT值及回声强度均逐渐增高(F=146.14、16.74,P均<0.01)。结论 FBS-PFH-NPs兼具靶向Hela细胞及CT/超声双模态显像能力。
英文摘要:
      Objective To prepare folate-targeted phase-transition nanoparticles carrying bismuth sulfide (FBS-PFH-NPs), in order to use for targeting performance and CT/ultrasound (US) imaging in vitro. Methods Rotary evaporator and probe-type sonication methods were used to prepare FBS-PFH-NPs. Basic characteristics of FBS-PFH-NPs were detected using several analytical methods. The targeting performance of FBS-PFH-NPs was verified through incubation with cervical cancer Hela cells in vitro. The echo intensity and temperature variation of FBS-PFH-NPs were observed after irradiating with HIFU using different acoustic powers (60, 90, 120, 150 and 180 W), respectively. The effects of FBS-PFH-NPs with different Bi2S3 concentrations (1.0, 2.0, 3.0, 4.0 and 5.0 mg/ml) for enhancing CT/US imaging were investigated. Results FBS-PFH-NPs were prepared with diameter (458.50±69.22)nm, which showed regular spherical morphology and uniform size under microscope. Bi2S3 nanoparticles randomly distributed in the lipid shell, and the concentration of Bi2S3 was 1.0 mg/ml. A mass of FBS-PFH-NPs was bond to Hela cells. The phase-transition of FBS-PFH-NPs occurred after irradiation with HIFU. The echo intensities and temperature of FBS-PFH-NPs gradually enhanced with increased HIFU powers (F=110.09, 440.69, both P<0.01). The echo intensities and CT value of FBS-PFH-NPs gradually enhanced with increased Bi2S3 concentrations (F=146.14, 16.74, both P<0.01). Conclusion FBS-PFH-NPs can not only target to Hela cell specifically, but also be applied in CT/US dual-modal imaging in vitro.
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