岳元勋,刘影,任志忠,黄鑫,杨晓伟,张跃伟.明胶海绵微粒TACE治疗Barcelona临床肝癌分期B期肝细胞癌对外周血中髓系来源抑制性细胞的影响[J].中国介入影像与治疗学,2019,16(5):280-284
明胶海绵微粒TACE治疗Barcelona临床肝癌分期B期肝细胞癌对外周血中髓系来源抑制性细胞的影响
Influence of gelatin sponge microparticles-TACE on myeloid-derived suppressor cells in peripheral blood of patients with Barcelona clinic liver cancer classification stage B hepatocellular carcinoma
投稿时间:2019-01-09  修订日期:2019-03-25
DOI:10.13929/j.1672-8475.201901018
中文关键词:  癌,肝细胞  Barcelona临床肝癌分期  明胶海绵微粒  栓塞,治疗性  髓系来源抑制性细胞  免疫微环境
英文关键词:carcinoma, hepatocellular  Barcelona clinic liver cancer classification  gelatin sponge microparticles  embolization, therapeutic  myeloid-derived suppressor cells  immunomicroenvironment
基金项目:国家自然科学基金(81571783)、北京市医院管理局2017年度临床医学发展专项——"扬帆"计划(ZYLX201712)。
作者单位E-mail
岳元勋 大连大学中山临床学院, 辽宁 大连 116001  
刘影 清华大学附属北京清华长庚医院肝胆介入科, 北京 102218  
任志忠 清华大学附属北京清华长庚医院肝胆介入科, 北京 102218  
黄鑫 清华大学附属北京清华长庚医院肝胆介入科, 北京 102218  
杨晓伟 清华大学附属北京清华长庚医院肝胆介入科, 北京 102218  
张跃伟 清华大学附属北京清华长庚医院肝胆介入科, 北京 102218 zhangyuewei1121@sina.com 
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中文摘要:
      目的 探讨应用明胶海绵微粒TACE(GSMs-TACE)治疗Barcelona临床肿瘤(BCLC)分期B期肝细胞癌(HCC)对患者外周血中髓系来源抑制性细胞(MDSCs)的影响。方法 对5例临床确诊为BCLC B期的HCC患者(HCC组)行GSMs-TACE治疗。采用流式细胞仪分别检测患者术前、术后10天及术后30天外周血中MDSCs频率(即MDSCs细胞团占HLA-DR-细胞群的比例)。另收集7名健康志愿者(正常对照组),于HCC组术前同期进行MDSCs频率检测。比较HCC患者术前及术后不同时间MDSCs频率的差异及HCC组术前与正常对照组同期MDSCs频率的差异。结果 HCC组GSMs-TACE术前外周血中MDSCs频率为(30.26±12.12)%,术后10天降至(10.22±3.79%),术后30天降至(7.33±3.38)%,总体差异有统计学意义(P < 0.001);两两比较显示术后30天(P < 0.001)及术后10天(P=0.011)均明显低于术前。HCC组术前MDSCs频率明显高于正常对照组同期水平[(30.26±12.12)% vs(3.41±1.89)%,t=5.876,P < 0.001)。结论 BCLC B期HCC患者经GSMs-TACE治疗后外周血中MDSCs频率显著降低;GSMs-TACE对患者的机体免疫功能具有正向调节作用。
英文摘要:
      Objective To investigate the influence of gelatin sponge microparticles-TACE (GSMs-TACE) on myeloid-derived suppressor cells (MDSCs) in peripheral blood of patients with Barcelona clinic liver cancer (BCLC) classification stage B hepatocellular carcinoma (HCC). Methods Five patients with clinically diagnosed BCLC B-stage HCC (HCC group) underwent GSMs-TACE. Flow cytometry was used to detect the frequency of MDSCs (the proportion of MDSCs clusters to HLA-DR- cell) in the peripheral blood of patients before GSMs-TACE and 10 days as well as 30 days after operation, respectively. Seven healthy volunteers (normal control group) were enrolled. The MDSCs frequency of normal control group was detected simultaneously with HCC group before GSMs-TACE. Statistical analysis was performed to compare the differences of the frequency of MDSCs in HCC patients before and after GSMs-TACE. And the frequency of MDSCs of HCC group was compared with that of normal control group. Results The frequency of MDSCs in peripheral blood of patients with HCC before GSMs-TACE was (30.26±12.12)%, which decreased to (10.22±3.79)% after 10 days and decreased to (7.33±3.38)% after 30 days (P < 0.001). Pairwise comparison showed that the frequency of MDSCs at 30 days (P < 0.001) and 10 days (P=0.011) after GSMs-TACE was lower than that before operation,respectively. The frequency of preoperative MDSCs of HCC group was statistically higher than that of normal control group ([30.26±12.12]% vs[3.41±1.89]%, t=5.876, P < 0.001). Conclusion The frequency of MDSCs in peripheral blood of patients with BCLC B-stage HCC significantly reduced after GSMs-TACE treatment. GSMs-TACE treatment has positive regulation effect on the immune function of patients.
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