| 刘祝,肖如辉,赵艳芬,张治涛,杜勇.铁沉积对非酒精性脂肪性肝炎兔模型肝脏质子密度脂肪分数的影响[J].中国介入影像与治疗学,2024,21(4):232-236 |
| 铁沉积对非酒精性脂肪性肝炎兔模型肝脏质子密度脂肪分数的影响 |
| Impact of iron deposition on liver proton density fat fraction in non-alcoholic steatohepatitis rabbit models |
| 投稿时间：2024-01-12 修订日期：2024-03-01 |
| DOI：10.13929/j.issn.1672-8475.2024.04.010 |
| 中文关键词: 兔 非酒精性脂肪肝 脂肪组织 铁 磁共振成像 |
| 英文关键词:rabbits non-alcoholic fatty liver disease adipose tissue iron magnetic resonance imaging |
| 基金项目:国家临床重点专科建设项目（川卫医政函［2023］87号） |
|
| 摘要点击次数: 137 |
| 全文下载次数: 149 |
| 中文摘要: |
| 目的 建立兔非酒精性脂肪性肝炎（NASH）模型，观察铁沉积对肝脏质子密度脂肪分数（PDFF）的影响。方法 将70只新西兰大白兔随机分为单纯高脂组（A组，n=35）及高脂联合铁剂组（B组，n=35）；再将B组分为1、2、3、4、8、12及16 ml共7个亚组，每组5只。对A组以高脂饲料饲养，对B组以高脂饲料饲养并经耳缘静脉注射右旋糖酐铁溶液（含铁100 mg/ml），建立NASH模型；行MRI及病理学检查，观察基于MRI所测PDFF与基于病理所测脂肪分数（FF）的相关性。结果 建模结束时A、B组兔存活率分别为91.43%和74.29%，建模用时分别为（7.27±1.01）周和（5.07±1.27）周。B组各亚组中，随注射铁含量增加，MRI显示肝脏信号逐渐减低、胆囊及大血管显示欠清，其中 1及2 ml亚组拟合优度分数均＜5%、其余各亚组均≥5%，且仅1及2 ml亚组PDFF与FF相关（r=0.961、0.893，P=0.039、0.041）。结论 NASH模型兔肝脏铁沉积增加将影响MRI测量肝脏PDFF的准确性。 |
| 英文摘要: |
| Objective To establish rabbit non-alcoholic steatohepatitis （NASH） model, and to observe the impact of iron deposition on liver proton density fat fraction （PDFF）. Methods Seventy New Zealand white rabbits were randomly divided into simple high-fat group （group A, n=35） and high-fat combined iron group （group B, n=35）, then those in group B were further divided into 7 subgroups （each n=5）. NASH models were established through simply feeding with high-fat feed in group A, or combined with injecting iron dextran solution （containing 100 mg/ml iron） 1, 2, 3, 4, 8, 12 and 16 ml, respectively. MRI and pathological examinations were performed, and the correlation between PDFF measured with MRI and fat fraction（FF）measured with pathology was analyzed. Results After modeling, the survival rates of rabbits in group A and B was 91.43% and 74.29%, respectively, with modeling time of （7.27±1.01） and （5.07±1.27） weeks, respectively. In group B, with the increase of the iron content injection, the liver signal on MRI gradually decreased, and the gallbladder and large blood vessels became not clear, the goodness of fit scores of 1 and 2 ml subgroups were both ＜5%, while of the other 5 subgroups were≥5%. PDFF was correlated with FF in 1 and 2 ml subgroups （r=0.961, 0.893； P=0.039, 0.041）. Conclusion The increased liver iron deposition could impact the accuracy of MRI measurement of PDFF in rabbit NASH models. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|
