廖海芬,孟文,牛丽丽,潘敏.以低强度聚焦超声刺激大鼠迷走神经的安全性[J].中国介入影像与治疗学,2024,21(6):358-362
以低强度聚焦超声刺激大鼠迷走神经的安全性
Safety of low-intensity focused ultrasound for stimulating rat vagus nerve
投稿时间:2024-05-06  修订日期:2024-05-21
DOI:10.13929/j.issn.1672-8475.2024.06.010
中文关键词:  迷走神经  大鼠  低强度聚焦超声
英文关键词:vagus nerve  rats  low-intensity focused ultrasound
基金项目:国家自然科学基金项目(T2122023)。
作者单位E-mail
廖海芬 广州中医药大学深圳医院(福田)超声科, 广东 深圳 518034
中国科学院深圳先进技术研究院劳特伯生物医学成像研究中心, 广东 深圳 518055 
 
孟文 中国科学院深圳先进技术研究院劳特伯生物医学成像研究中心, 广东 深圳 518055  
牛丽丽 中国科学院深圳先进技术研究院劳特伯生物医学成像研究中心, 广东 深圳 518055  
潘敏 广州中医药大学深圳医院(福田)超声科, 广东 深圳 518034 min.pan@siat.ac.cn 
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中文摘要:
      目的 观察以低强度聚焦超声(LIFU)刺激大鼠迷走神经的安全性。方法 将12只雄性SD大鼠随机均分为刺激A、B、C组和假刺激组(D组),每组3只。以装配适宜准直器的超声刺激系统,分别以1.05、3.87及4.25 MPa声压对A、B、C组大鼠左侧迷走神经行LIFU刺激,脉冲重复频率、占空比及刺激方式均相同;对D组大鼠将超声换能器定位于左侧颈部但不予刺激。记录LIFU刺激前、中、后4组大鼠心率及颈部局部温度变化。结束刺激后对C、D组采集MR T2WI,取迷走神经,观察有无病理改变。结果 LIFU刺激前5 min、刺激中及刺激后5 min,4组间心率差异均无统计学意义(P 均>0.05)。A、B、C、D组换能器下方局部区域温度分别为28.1~30.1 ℃、28.3~30.3 ℃、27.9~30.4 ℃及27.8~30.2 ℃,温度波动均小于2.5 ℃。T2WI显示C、D组大鼠颈部软组织无明显异常;2组大鼠迷走神经均未见明显病理改变。结论 以特定参数LIFU刺激大鼠迷走神经的安全性较好。
英文摘要:
      Objective To observe the safety of low-intensity focused ultrasound (LIFU) for stimulating rat vagus nerve. Methods Twelve male SD rats were randomly divided into stimulation group A, B, C and sham stimulation (D) group (each n=3). A LIFU stimulation system equipped with a suitable collimator was used to stimulate rat left vagus nerve in A, B, C groups, with acoustic pressure of 1.05, 3.87 and 4.25 MPa, respectively, with the same pulse repetition frequency, duty ratio and stimulation mode. For rats in group D, the ultrasonic transducer was positioned on the left side of the neck but no stimulation was performed. The changes of heart rate and local neck temperature were recorded before, during and after LIFU stimulation. After stimulation, neck MR T2WI were acquired for group C and D. Then pathological examination of vagus nerve was performed to observe whether there were abnormal changes after stimulation. Results No significant difference of heart rate 5 min before, during and 5 min after stimulation was found among 4 groups (all P>0.05). The local temperature beneath the transducer of rats in group A, B, C and D ranged from 28.1—30.1 ℃, 28.3—30.3 ℃, 27.9—30.4 ℃ and 27.8—30.2 ℃, respectively, and the temperature fluctuation all less than 2.5 ℃. T2WI showed no obvious abnormality of neck soft tissue in group C nor D, while no significant pathological changes of rat vagus nerve was detected. Conclusion LIFU with proper parameters was safe for stimulating rat vagus nerve.
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