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吴敬玺,梁业,任益功,袁冰,段峰.经导管肝动脉栓塞联合腹腔化疗调控肝内胆管癌大鼠模型CD4⁺及CD8⁺T细胞浸润[J].中国介入影像与治疗学,2025,22(12):796-800

经导管肝动脉栓塞联合腹腔化疗调控肝内胆管癌大鼠模型CD4⁺及CD8⁺T细胞浸润

Regulatory effect of transcatheter hepatic artery embolization combined with intraperitoneal chemotherapy on CD4⁺ and CD8⁺ T cell infiltration in rat models of intrahepatic cholangiocarcinoma

投稿时间：2025-11-28 修订日期：2025-12-03

DOI：10.13929/j.issn.1672-8475.2025.12.009

中文关键词: 胆管癌栓塞,治疗性抗肿瘤联合化疗方案 CD4阳性T淋巴细胞 CD8阳性T淋巴细胞

英文关键词:cholangiocarcinoma embolization,therapeutic antineoplastic combined chemotherapy protocols CD4-positive T-lymphocytes CD8-positive T-lymphocytes

基金项目:国家自然科学基金（U24A20760）。

作者	单位	E-mail
吴敬玺	解放军医学院, 北京 100853
梁业	中国人民解放军联勤保障部队第904医院超声诊断科, 江苏无锡 214044
任益功	中国人民解放军总医院肿瘤医学部, 北京 100039
袁冰	中国人民解放军总医院肿瘤医学部, 北京 100039
段峰	解放军医学院, 北京 100853 中国人民解放军总医院肿瘤医学部, 北京 100039	duanfeng@vip.sina.com

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中文摘要:

目的构建大鼠原位肝内胆管癌（ICC）模型，观察经导管肝动脉栓塞（HAE）联合吉西他滨+顺铂（GC）腹腔化疗对CD4⁺及CD8⁺ T细胞浸润的调控作用。方法以硫代乙酰胺（TAA）诱导40只SD大鼠构建原位ICC模型。将24只至少存在1个最大径0.5～1.5 cm肿瘤的大鼠随机均分为联合组（HAE+GC）、GC组、HAE组及空白对照组，每组各6只。采用流式细胞仪检测肿瘤组织的CD4⁺T细胞在CD3⁺T细胞中和CD8⁺T细胞在CD3⁺T细胞中的占比，以免疫荧光染色法检测肿瘤组织中的CD4⁺T细胞、CD8⁺T细胞密度并进行组间比较。结果联合组、GC组、HAE组及对照组CD4⁺T细胞在CD3⁺T细胞中的占比分别为（19.60±3.68）%、（18.28±2.85）%、（33.98±5.49）%及（29.31±2.57）%，CD8⁺T细胞在CD3⁺T细胞中的占比分别为（27.03±7.99）%、（18.51±4.02）%、（28.35±3.91）%及（24.30±2.20）%，总体及两两比较差异均无统计学意义（P均＞0.05）。联合组、GC组及HAE组CD4⁺T细胞及CD8⁺T细胞密度均显著低于对照组（P均＜0.05）；联合组CD4⁺T细胞密度及GC组CD8⁺T细胞密度均显著低于HAE组（P=0.003、0.007）。结论 HAE用于治疗ICC对保留CD8⁺T细胞比例及浸润密度具有一定优势；联合GC可差异化调控CD4⁺和CD8⁺T细胞比例及浸润密度以调控T细胞亚群。

英文摘要:

Objective To establish intrahepatic cholangiocarcinoma （ICC） in situ rat models， and to observe the regulatory effect of transcatheter hepatic artery embolization （HAE） combined with intraperitoneal chemotherapy with gemcitabine plus cisplatin （GC） on infiltration of CD4⁺ and CD8⁺ T cells. Methods ICC in situ were induced in 40 SD rats using thioacetamide （TAA）. Ultimately， 24 rats with at least one 0.5—1.5 cm tumor were selected and randomly divided into combined （HAE+GC） group， GC group， HAE group and control group （each n=6）. The proportion of CD4⁺ T cells and CD8⁺ T cells in CD3⁺ T cells of tumor were measured with flow cytometry， the density of CD4⁺ and CD8⁺ T cells in tumor were assessed using immunofluorescence staining and compared among groups. Results The proportion of CD4⁺ T cells in CD3⁺ T cells in combined group， GC group， HAE group and control group was （19.60±3.68）%，（18.28±2.85）%，（33.98±5.49）% and （29.31±2.57）%， respectively， of CD8⁺ T cells in CD3⁺ T cells was （27.03±7.99）%，（18.51±4.02）%，（28.35±3.91）% and （24.30±2.20）%，respectively， no significant difference was found among 4 groups nor in pairwise comparisons （all P＞0.05）. The density of both CD4⁺ and CD8⁺ T cells in combined group， in GC group and HAE group were all significantly lower than those in control group （all P＜0.05）. CD4⁺ T cell density in combined group and CD8⁺ T cell density in GC group were significantly lower than those in HAE group （P=0.003， 0.007）. Conclusion HAE demonstrated certain advantages in preserving the proportion and infiltration density of CD8⁺ T cells for treating ICC. Combining HAE and GC could modulate T cell subsets through differentially regulating the proportions and infiltration densities of CD4⁺ and CD8⁺ T cells.

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