| 莫海畲,高端贵,邓钰琳,宋具鸿,李玥,李俊祥,蔡燕.亚致死热处理联合帕博利珠单抗杀灭甲状腺乳头状癌肿瘤细胞[J].中国介入影像与治疗学,2026,23(5):292-299 |
| 亚致死热处理联合帕博利珠单抗杀灭甲状腺乳头状癌肿瘤细胞 |
| Sublethal hyperthermia combined with pembrolizumab for killing tumor cells of papillary thyroid carcinoma |
| 投稿时间：2026-02-19 修订日期：2026-04-14 |
| DOI：10.13929/j.issn.1672-8475.2026.05.008 |
| 中文关键词: 甲状腺肿瘤 消融技术 程序性细胞死亡受体1 |
| 英文关键词:thyroid neoplasms ablation techniques programmed cell death 1 receptor |
| 基金项目:贵州省科技计划项目（黔科合基础-ZK［2023］一般 356）。 |
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| 中文摘要: |
| 目的 观察亚致死热处理联合帕博利珠单抗杀灭甲状腺乳头状癌（PTC）肿瘤细胞的价值。方法 将TPC-1细胞分为亚致死热处理联合帕博利珠单抗组（联合组）、亚致死热处理组（热处理组）、帕博利珠单抗组（单抗组）及对照组，分别予以相应处理，对比各组细胞活力、迁移能力、凋亡率及DNA损伤。将TPC-1细胞与T细胞共同培养，检测T细胞活化/效应指标、细胞因子/趋化因子；以蛋白质印迹法检测免疫原性相关蛋白。结果 联合组细胞活力及迁移能力最差、凋亡率最高，DNA损伤应答通路被明显激活，T细胞活化/效应指标阳性占比及细胞因子/趋化因子分泌水平均最高，免疫原性相关蛋白表达显著上调；热处理组及单抗组次之，再次为对照组。结论 亚致死热处理联合帕博利珠单抗有助于杀灭TPC-1细胞。 |
| 英文摘要: |
| Objective To observe the value of sublethal hyperthermia combined with pembrolizumab for killing tumor cells of papillary thyroid carcinoma （PTC）. Methods TPC-1 cells were divided into combined group （sublethal hyperthermia combined with pembrolizumab）， hyperthermia group （sublethal hyperthermia）， pembrolizumab group and control group， each group received corresponding management. The cell viability， migration ability， apoptosis rate and DNA damage were assessed. Then TPC-1 cells were co-cultured with T cells to detect T cell activation/effector indexes and cytokines/chemokines. The immunogenicity-related proteins were detected using Western blot. Results Cell viability and migration ability were the worst， the apoptosis rate was the highest， DNA damage response pathway was significantly activated， the positive proportion of T cell activation/effector indexes and the secretion level of cytokines/chemokines were the highest， and the expression of immunogenicity-related proteins was significantly upregulated in combined group， which were all the second in hyperthermia group and pembrolizumab group and the third in control group. Conclusion Sublethal hyperthermia combined with pembrolizumab was helpful for killing TPC-1 cells. |
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